Skip to main content
. Author manuscript; available in PMC: 2013 Sep 19.
Published in final edited form as: Nature. 2012 Mar 28;484(7394):376–380. doi: 10.1038/nature10959

Figure 1. Locally generated glia as a major source of astrocytes.

Figure 1

a, Procedure to label SVZ/radial glia-derived astrocytes by electroporation. b, The distribution of astrocytes (arrows) 2 weeks after electroporation. VZ, ventricular zone. c, Percentages of astrocytes at different locations. WM, white matter. d, Proliferating cells (Ki67+, red) in a cortical section of P3 mouse. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, blue). e, Procedure to label locally proliferating cells by retrovirus. f, Cells labelled by retrovirus (green). g, Image of infected astrocytes. Astrocytes (BLBP+, red) with GFP (GFP+BLBP+) or without GFP (GFP−BLBP+) in the outlined region (dashed line) were included for analysis in h. RV, retrovirus. h, Percentages of astrocytes labelled by retrovirus injected locally, calculated as 100 ×(BLBP+GFP+ cells/BLBP+ cells). Scale bars, 200 μm (b), 50 μm (d), 500 μm (f) and 40 μm (g).