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. Author manuscript; available in PMC: 2013 Sep 19.
Published in final edited form as: J Comp Neurol. 2012 Oct 15;520(15):3528–3552. doi: 10.1002/cne.23104

Figure 4. Molecular markers in the central canal.

Figure 4

A–C) Most central canal cells express vimentin intermediate filaments (magenta). Some cells also contain GFAP (green, arrows). GFAP is also frequent outside the canal. Some cells also express GFAP and vimentin in long processes (arrowheads). D–F) Nearly all canal cells express CD24 (magenta). Arrows point to a CD24- cell expressing GFAP (green). G–I) Nestin (magenta) was expressed predominantly in the dorsal and ventral regions of the central canal, especially in very long dorsal radial processes, which were frequently also positive for GFAP (green, arrowheads). The arrows point to a nestin+ cell also labeled with GFAP. J–O) Expression of FoxJ1 in the FoxJ1 ::Cre;Z/EG mice. J–L) FoxJ1 (green) is also expressed by vimentin+ cells (magenta). M–O) Immunofluorescence for GFP (green) and CD24 (magenta). Nuclei are stained with DAPI (blue). Scale bar in A (A–L), 20 µm; M (M–O), 10 µm.