Table 1.
Primary antibodies used in this study.
| Antigen | Immunogen | Manufacturer, species, catalog number | Dilution used |
|---|---|---|---|
| BrdU | BrdU conjugated to bovine seroalbumin | Dako (Glostrup, Denmark), mouse monoclonal IgG, #M0744 | (1:150) |
| BrdU | BrdU | Abcam (Cambridge, MA), rat monoclonal IgG, #ab6326 | (1:200) |
| GFAP | GFAP isolated from cow spinal cord | Dako, rabbit polyclonal IgG, #Z0334 | (1:1000) |
| Vimentin | Human thymic nuclear extract | Sigma-Aldrich (St. Louis, MO), mouse monoclonal (clon LN6) IgM, #V- 2258 |
(1:1000) |
| CD24 | C57BL/10 Mouse splenic T Lymphocytes | BD Biosciences (Franklin Lakes, NJ), rat monoclonal (clon M1/69) IgG2b, #557436 |
(1:500) |
| Phospho-histone H3 | KLH-conjugated peptide (ARK[pS]TG- GKAPRKQLC) corresponding to amino acids 7–20 of human histone H3 |
Millipore (Billerica, MA), rabbit polyclonal IgG, #06–570 | (1:500) |
| Ki67 | Prokaryotic recombinant fusion protein corresponding to a 1086bp Ki67 motif- containing cDNA fragment |
Novocastra (Newcastle Upon Tyne, United Kingdom), mouse monoclonal (clone MM1) IgG1, #NCL-L-Ki67-MM1 |
(1:500) |
| γ-tubulin | Synthetic peptide corresponding to the N-terminal region of human γ-tubulin (aa 38–53, with C-terminally added lysine) conjugated to KLH |
Sigma-Aldrich, rabbit polyclonal, #T5192 | (1:1000) |
| γ-tubulin | Synthetic peptide conjugated to KLH, corresponding to N-terminal aa 38–53 of γ-tubulin |
Abcam, mouse monoclonal [GTU-88] IgG1, #AB11316 | (1:500) |
| β-catenin | Mouse β-catenin aa 571–781 | BD Biosciences, mouse monoclonal (clone 14) IgG1, #610153 | (1:500) |
| β-catenin | Synthetic peptide corresponding to aa 768–781 of human or mouse β-catenin conjugated to KLH as immunogen |
Sigma-Aldrich, Rabbit polyclonal IgG, #C2206 | (1:1000) |
| GFP | Purified recombinant green fluorescent protein | Aves Labs (San Diego, CA), chicken polyclonal IgY, #GFP-1020 | (1:200) |
| NeuN | Purified cell nuclei from mouse brain | Millipore, mouse monoclonal IgG (clone A60), #MAB377 | (1:500) |
| DCX | Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of human doublecortin; sequence YLPLSLDDSDSLGDSM |
Abcam, rabbit polyclonal IgG, #AB18723 | (1:500) |
| Nestin | Nestin purified from embryonic rat spinal cord | Millipore, rat monoclonal IgG1, clone Rat-401, #MAB353 | (1:200) |
| Sox2 | Peptide corresponding to amino acids 249–264 of human Sox2 (SSSPPVVTSSSHSRAP; identical to mouse), affinity-purified serum |
Millipore, rabbit polyclonal IgG, #AB5603 | (1:200) |
| CD133 | Embryonic mouse telencephalic neuroepithelium | eBioscience (San Diego, CA) rat monoclonal IgG1, #14–1331 | (1:100) |