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. Author manuscript; available in PMC: 2013 Sep 19.
Published in final edited form as: Hum Mol Genet. 2007 Nov 27;17(5):747–758. doi: 10.1093/hmg/ddm347

Figure 4.

Figure 4

EMSA and promoter assays using rs3756450 at SLC6A3. (A) Nuclear extracts from SHSY-5Y cells were incubated with labeled probes. The labeled probe for the T allele was loaded in the first three lanes, and the labeled probe for the C allele in the next three lanes. Unlabeled competitor oligonucleotides were included in 50-fold molar excess in lanes 3 and 6. Lanes 1 and 4 indicate the migration of the labeled probe without the nuclear extract. Asterisk indicates altered band shift pattern for T allele of rs3756450 (lane 2) with respect to C allele (lane 5), despite 2-fold excess of nuclear extract added to lanes 5 and 6. (B) Promoter activity in a dual-luciferase assay system for constructs containing either the C or T allele at rs3756450 but identical at all other bases.