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. Author manuscript; available in PMC: 2013 Sep 19.
Published in final edited form as: Biochim Biophys Acta. 2012 May 10;1823(8):1306–1315. doi: 10.1016/j.bbamcr.2012.05.003

Fig. 5. Effect of cycloheximide (CHX) on 2ME2-induced promataphase arrest.

Fig. 5

Cells were pre-treated for 2 h with cycloheximide (1 μg/mL) and then treated for additional 12 h with 2 μM 2ME2. Total cell lysates were analyzed by Western blotting for cyclin B1 and Cdc2 protein levels (A, upper panel). The relative protein levels of cyclin B1 and Cdc2 were calculated according to their densitometry readings, which were normalized to GAPDH protein level (A, lower panel). Each value is mean ± S.D. from triplicate measurements. * P < 0.05 versus vehicle control; #P < 0.05 versus 2ME2 treatment. The gross nuclear morphology was examined under fluorescence microscopy (original magnification, × 200) after the cells were stained with Hoechst-33342 (B, upper panel). The arrow points to a prometaphase-arrested cell. The quantitative data (B, lower panel) was based on counting 200 nuclei in each treatment group. Each bar is the mean ± S.D. from triplicate determinations. * P < 0.05 versus vehicle control; #P < 0.05 versus 2ME2 treatment.