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. Author manuscript; available in PMC: 2013 Sep 19.
Published in final edited form as: J Endocrinol. 2009 Dec 23;204(3):287–297. doi: 10.1677/JOE-09-0183

Figure 3. cAMP/PKA signaling mediates the effects of CaR activation on PTHrP and ACTH secretion.

Figure 3

A) PTHrP levels in conditioned media harvested from AtT-20 cells pretreated with the PKA inhibitor, H89 (10 μM), prior to exposure to high calcium (5 mM) or neomycin. H89 lowered basal rates of PTHrP secretion and blocked the response to high calcium or neomycin. B) ACTH levels in conditioned media from cells pretreated with H89 (10 μM), prior to exposure to high calcium (5 mM) or neomycin. As with PTHrP, inhibition of PKA lowered basal secretion of ACTH and blocked the effects of calcium or neomycin. Data are means ± S.E. from three independent experiments. Statistical significance (p<0.01 or lower) is noted on the graph: a denotes a significant difference as compared with 0.1mM calcium, b denotes a significant difference as compared with 5mM calcium and c denotes a significant difference as compared to neomycin.