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. Author manuscript; available in PMC: 2013 Dec 24.
Published in final edited form as: Nat Commun. 2013;4:2037. doi: 10.1038/ncomms3037

Figure 2. The combination of contextual sequence and position determines the preference of phosphotyrosines for cis-interaction.

Figure 2

(a) The models depict the cis-interactions of the Y580F, Δ580 and Δ542 reporters upon phosphorylation in vitro. (b) The ratio time courses of Δ542 and Δ580 reporters. Y542 and Y580 sequences are colored in red and green, respectively. (c) The binding curves of the C-SH2 domain (4.5 μM) towards immobilized pY542 or pY580 peptides measured by SPR (refer to Supplementary Figure S8 for the binding kinetics and original sensorgrams). (d) The ratio time courses of engineered Y542Y542 and its mutant reporters as indicated.