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. Author manuscript; available in PMC: 2013 Sep 19.
Published in final edited form as: Mol Cell. 2006 Aug 4;23(3):389–399. doi: 10.1016/j.molcel.2006.05.043

Figure 3.

Figure 3

Spliceosome Disassembly Requires the DExD/H-box ATPase Brr2p (A) Disassembly of Prp43p-associated spliceosomes is inhibited by brr2 mutations. Spliceosomes were purified from the wild-type strain BRR2 (yJPS999) or the mutant strains brr2-1 (yJPS1000) and brr2-R1107A (yJPS1001). Spliceosomes were incubated and analyzed as in Figure 2B. (B) As for brr2-1, the mutant brr2-R1107A is cold sensitive. Cells were diluted from liquid culture and grown on rich media for 2 days at 30°C or 6 days at 15°C. (C) In brr2 mutants, the excised lariat intron from U3A accumulates. Wild-type BRR2 (yJPS999) or the mutants brr2-1 (yJPS1000) and brr2-R1107A (yJPS1001) were grown in rich liquid media at 30°C and shifted to 15°C for 4 h. RNA was analyzed by Northern as in Figure 1B.