Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Jan;31(1):36–44. doi: 10.5732/cjc.011.10317

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Chinese Journal of Cancer

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resulting work under the same or similar license to this one. The work must be attributed back to the original author and commercial use is not permitted without specific permission.

PMC Copyright notice

Figure 2. — A, colony formation assay (upper panel) and anchorage-independent growth of 5-8F and SUNE2 cells in soft agar (lower panel). SUNE2 cells form bigger and more colonies compared with 5-8F cells. B, quantitative analysis of colony numbers of 5-8F cells and SUNE2 cells with three independent assays. ***, P < 0.001. C, significant tumor formation in a nude mouse after injection with SUNE2 cells at passage 6. D, a paraffin section of a xenograft formed by SUNE2 cells was stained with HE (×400), showing obvious tumor nests, oval nuclei, and indistinct cell borders. E, a paraffin section was stained with monoclonal anti-keratin antibody (Zymed), and only the epithelial cells were stained brown in cytoplasm (×100). F, a representative field is shown by keratin staining of the xenograft (×400).