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. 2012 Jun;31(6):287–294. doi: 10.5732/cjc.011.10376

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Chinese Journal of Cancer

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resulting work under the same or similar license to this one. The work must be attributed back to the original author and commercial use is not permitted without specific permission.

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Figure 3. — A, representative experiments illustrate the EBV-specific cytotoxic activity of TILs from three different NPC patients to target a panel of cells, including auto-LCL (EBV⁺), auto-blast cells (EBV⁻), NPC tumor cell line C666 (EBV⁺), and leukemia cell line K562 (EBV⁻). The effector cell and target cell panels were co-cultured at different ratios for 5 h, and target cells were labeled by 5- or 6-(N-Succinimidyloxycarbonyl)-3′,6′-O,O′-diacetylfluorescein (CFSE). The percentage of specific lysis of target cells was determined by 7-amino-actinomycin D (7-AAD) staining and fluorescence-activated cell sorting (FACS) detection. One of three experiments is shown in the figure. B, the average percentage of specific lysis of TILs to target cells, including auto-LCLs and auto-blast cells (n = 15), is shown in the figure. Error bars represent standard deviation.