Figure 5. FAN1 may play a crucial role during mitotic exit.

U2OS cells were transfected with FAN1-siRNA or scrambled siRNA for 48 h (A) or with the indicated plasmids for 24 h (B). Then, the cells were synchronized in M phase by incubation with 100 ng/mL nocodazole for 16 h. M phase cells selected by shake-off were released at indicated time points. Cells were collected for analysis by flow cytometry (left) and western blotting (right). Note: The percentages of G₂/M phase cells are indicated.