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. 2012 Aug;31(8):373–380. doi: 10.5732/cjc.012.10077

Figure 4. Validation of CHO cell lines. Clonogenic assays were performed in paired CHO lines.

Figure 4.

A, Rad51D-deficient 51D1 (◊) and Rad51D-complemented 51D1.3 (♦); B, wild-type AA8 (•) and XRCC3-deficient irs1SF (○). After 24 h of plating, cells were exposed to a range of concentrations of mitomycin C (MMC) for 24 h. Then, cells were incubated for an additional 4 days (51D1, 51D1.3, and AA8) or 6 days (irs1SF) after washing out MMC to allow colonies to grow. Each point on the survival curves is the mean ± SD of triplicates.