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. 2012 Aug;31(8):364–372. doi: 10.5732/cjc.012.10086

Figure 1. Etoposide and camptothecin induce apoptosis in undifferentiated but not neuronally differentiated SH-SY5Y cells.

Figure 1.

A, SH-SY5Y (simplified as SY5Y in the figure) cells were pretreated with KU-55933 (10 µmol/L) for 1 h before the addition of etoposide (Eto, 10 µmol/L) for another 3 h. Cells were lysed, and cell lysates were subjected to SDS-PAGE and Western blotting for the detection of PARP, Caspase-3, N-CAM, and β-actin. N-CAM is a marker of post-mitotic neuron-like cells, and β-actin is a loading control. B, cells were pretreated with KU-55933 (10 µmol/L) for 1 h before the addition of camptothecin (CPT, 10 µmol/L) for another 3 h. Following cell lysis and SDS-PAGE, the same set of proteins were detected by immunoblotting as described above.