Skip to main content
. 2012 Aug;31(8):364–372. doi: 10.5732/cjc.012.10086

Figure 4. ATM is important for the survival of human neuron-like SH -SY5Y cells following growth factor deprivation.

Figure 4.

A, ATM may mediate Akt phosphorylation at Ser473 through PI3K in response to insulin in differentiated SH-SY5Y cells. Cells were serum-starved for 6 h and treated with mTOR inhibitor rapamycin (25 nmol/L), DNA-PK inhibitor NU7026 (10 µmol/L), or PI3K inhibitor LY294002 (16 µmol/L) for 1 h before insulin stimulation of another 45 min. Cells were then collected, and cell lysates were subjected to SDS-PAGE and Western blotting for the detection of phospho-Akt at Ser473 and β-actin. B, ATM inhibition by KU-55933 induces apoptosis in differentiated but not undifferentiated SH-SY5Y cells. Cells were starved for 6 h in serum-free medium. KU-55933 (10 µmol/L) was added 1 h before insulin (100 nmol/L) treatment. After 3 h of insulin stimulation, cells were collected, and cell lysates were subjected to SDS-PAGE and Western blotting for the detection of PARP, caspase-3, and β-actin.