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. Author manuscript; available in PMC: 2014 Aug 27.
Published in final edited form as: Biochemistry. 2013 Aug 14;52(34):5941–5951. doi: 10.1021/bi400541v

Scheme I.

Scheme I

Kinetic model for photostationary states in H93G-CO and P420-CO with H indicating a proximal histidine ligand and L indicating a water (or, on longer timescales a thiol) proximal ligand. The A-states have CO bound, the B-states have CO in the distal pocket, while CO has escaped into solution in the C-states. The H93G-CO sample reveals poplulations of AH, AL, and CL while only AH and CL populations are observed in P420-CO (unless the states AH and AL have identical FeCO frequencies). This kinetic scheme is reduced to an effective three state system and analyzed more completely in the Supporting Information.