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. Author manuscript; available in PMC: 2013 Sep 19.
Published in final edited form as: Clin Cancer Res. 2012 Jul 24;18(18):5031–5042. doi: 10.1158/1078-0432.CCR-12-0453

Figure 1.

Figure 1

Baseline expression and functional activity of EGFR and COX-2 in human pancreatic cancer cell lines. A, RNA was harvested from human pancreatic cancer cell lines and evaluated by qPCR for expression of COX-2 and EGFR; levels were normalized to β-actin internal loading control. B, the expression of EGFR, p-EGFR at tyr1068, and COX-2 was determined by Western blot analysis. Expression of β-actin was used as a loading control. C, the IC50 of erlotinib for inhibition of phosphorylation of EGFR after stimulation with 10 ng/mL EGF was determined by ELISA. D, PGE2 levels in conditioned media measured by ELISA after 24-hour incubation with apricoxib.