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. Author manuscript; available in PMC: 2013 Sep 19.
Published in final edited form as: Sci Transl Med. 2012 Jun 20;4(139):139ra85. doi: 10.1126/scitranslmed.3003921

Figure 3. Application of soluble rhMG53 increases membrane repair capacity in vitro.

Figure 3

(A) Isolated flexor digitorum brevis (FDB) muscle fibers from mg53-/- mice were treated with FM1-43 dye and 80 μg/mL MBP-MG53 or BSA, before injury by a UV laser (arrow). Scale bars, 20 μm. Degree of dye influx in multiple fibers is quantified as the change in fluorescent signal over baseline (ΔF/F0). Data are means ± SD (n represents individual muscle fibers). (B) Isolated FDB muscle fibers from dysferlin-/- mice were treated with 80 μg/ml MBP-MG53 or BSA before injury by a UV laser (arrow). Scale bars, 20 μm. Fluorescence influx data (ΔF/F0) for multiple fibers are shown on the right. Data are averages ± SD (n represents individual muscle fibers). Live imaging of this assay (movies S2 and S3) show entire time course of fluorescence influx after injury, from 0 to 200 seconds.