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. Author manuscript; available in PMC: 2014 Sep 17.
Published in final edited form as: FEBS Lett. 2013 Jul 23;587(18):3014–3020. doi: 10.1016/j.febslet.2013.07.025

Fig. 3.

Fig. 3

ERK is involved in nitric oxide production downstream of IFN-β and IL-6. (A) RAW264.7 cells were pre-treated with no inhibitor (no inhib), U0126, SP600125 or 2-aminopurine (2-amino) for 30 minutes and then treated with 10 ng/ml IFN-β for 24 hours or left untreated (control). (B, C) B10.s peritoneal macrophages (B) or RAW264.7 cells (C) were pre-treated with U0126 and then treated with 10 ng/ml IFN-β (B) or 10ng/ml IL-6 (C) for 24 hours or left untreated (control). The supernatant was removed and nitric oxide was analyzed by Greiss assay. Data are means +/− SEM of 3 to 4 samples per group.