FIG. 2.

Immunofluorescence antibody assay confirms expression of the recombinant proteins Gne and N in Sf9 cells. Monoclonal antibodies 4D4 and ID8 were used to detect expression of Gne and N, respectively. A specific green fluorescent signal around the nucleus of Gne-expressing cells is seen indicating that the recombinant protein is within the cell. Anti-Gn control, noninfected Sf9 cells stained with Gn monoclonal antibody (4D4); anti-N control, noninfected Sf9 cells stained with N monoclonal antibody (ID8) show negative staining. The cell nucleus is stained in blue color.