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. 2013 Sep;13(9):619–629. doi: 10.1089/vbz.2012.1285

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Copyright 2013, Mary Ann Liebert, Inc.

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FIG. 5. — Reactivity of recombinant RVFV proteins, nucleoprotein, N (A), nonstructural protein, NSs (B), glycoprotein, Gne (C), glycoprotein, Gc (D), and nonstructural protein NSm (E) with antisera from MP12 vaccinated sheep. Day 0, prevaccination sera; PC, positive control sera derived from sheep challenged with RVFV wild-type, ZH501; P1–P6, day-28 sera from sheep vaccinated with MP12 RVFV strain (Laramie, WY); P7–P10, day-28 sera from sheep infected with the wild-type virus (ZH501). Asterisks (*) denote level of statistical significance and show that differences in optical density (OD) values of sera tested for each of the time-points was significantly different (p<0.05) from day 0 (prevaccination) sera. Cutoff OD value for each enzyme-linked immunosorbent assay (ELISA) was determined by addition of 2 standard deviations to the mean OD value of serum obtained from prevaccinated/noninfected sheep. N, 0.320; NSs, 0.358; Gn, 0.395; Gc, 0.387; NSm, 0.208.

FIG. 5. — Reactivity of recombinant RVFV proteins, nucleoprotein, N (A), nonstructural protein, NSs (B), glycoprotein, Gne (C), glycoprotein, Gc (D), and nonstructural protein NSm (E) with antisera from MP12 vaccinated sheep. Day 0, prevaccination sera; PC, positive control sera derived from sheep challenged with RVFV wild-type, ZH501; P1–P6, day-28 sera from sheep vaccinated with MP12 RVFV strain (Laramie, WY); P7–P10, day-28 sera from sheep infected with the wild-type virus (ZH501). Asterisks (*) denote level of statistical significance and show that differences in optical density (OD) values of sera tested for each of the time-points was significantly different (p<0.05) from day 0 (prevaccination) sera. Cutoff OD value for each enzyme-linked immunosorbent assay (ELISA) was determined by addition of 2 standard deviations to the mean OD value of serum obtained from prevaccinated/noninfected sheep. N, 0.320; NSs, 0.358; Gn, 0.395; Gc, 0.387; NSm, 0.208.