Table 2.
Transcriptional response of RUNX-2 and cytoskeletal genes.
| Genes | 100Hz | 30Hz | ||||
|---|---|---|---|---|---|---|
| 0.15g | 1g | 2g | 0.15g | 1g | 2g | |
| RUNX-2 | 1.2 | 1.6 | 2.0 | 1.4 | 2.89 | 2.17 |
| ARHGEF11 | 1.3 | 2.5 | 2.5 | 1.3 | 5.6 | 6.0 |
| AURKC | 1.8 | 3.0 | 3.8 | 1.6 | 5.6 | 9.6 |
| CDC42 | 2.1 | 3.0 | 3.4 | 2.0 | 4.6 | 4.3 |
| CDK5R1 | 1.6 | 6.9 | 6.2 | 0.8 | 4.7 | 13.5 |
| CYFIP2 | 2.4 | 11.5 | 13.1 | 2.2 | 23.2 | 18.7 |
| LIM-K2 | 1.3 | 2.6 | 2.4 | 1.0 | 3.3 | 6.7 |
| MAP3K11 | 1.7 | 2.3 | 2.1 | 1.6 | 5.7 | 5.2 |
| MARK2 | 1.3 | 3.1 | 3.2 | 1.0 | 3.6 | 4.8 |
| NCK2 | 1.6 | 3.0 | 3.0 | 1.5 | 5.5 | 6.7 |
| PPP1R12B | 2.4 | 3.3 | 2.4 | 2.0 | 4.7 | 5.0 |
| RADIXIN | 2.7 | 4.2 | 3.8 | 2.0 | 3.9 | 3.7 |
| WAS | N/A | 26.8 | 40.0 | 6.9 | 27.6 | 43.0 |
| WASL | 2.1 | 2.6 | 2.8 | 1.9 | 4.7 | 3.1 |
|
| ||||||
| Functional Categories | ||||||
| Actin Remodeling | 1.6 | 3.8 | 4.5 | 1.8 | 5.5 | 5.9 |
| Calmodulin / Calcineurin | 1.2 | 1.9 | 1.8 | 1.2 | 4.1 | 3.4 |
| Cell Motility / Migration | 1.6 | 2.2 | 2.2 | 1.5 | 3.3 | 3.1 |
| Cell Projections | 1.9 | 3.3 | 3.4 | 1.8 | 5.3 | 4.5 |
| Cell Shape, Size, Polarity | 1.7 | 4.2 | 4.7 | 1.6 | 8.0 | 7.1 |
| Cytokinesis | 1.6 | 2.5 | 2.8 | 1.4 | 4.6 | 6.2 |
| Cytoskeleton Adaptor Activity: | 1.6 | 2.7 | 2.7 | 1.7 | 3.7 | 4.0 |
| G-Protein Signaling | 1.5 | 4.8 | 6.0 | 2.0 | 7.1 | 7.5 |
| Kinases & Phosphatases | 1.6 | 2.3 | 2.3 | 1.5 | 3.6 | 4.0 |
| Microtubules | 1.6 | 6.9 | 6.2 | 0.8 | 4.7 | 13.5 |
| Mean mRNA Activity | 1.6 | 3.5 | 3.7 | 1.5 | 5.0 | 5.9 |
After 7d of vibration in osteogenic medium, expression levels of RUNX-2 and 84 genes were determined. A total of 25 genes showed a greater than 2-fold response to at least one frequency/acceleration combination. In the upper panel, genes with a greater than 4-fold response are included. In the lower panel, the 25 genes were subdivided into 9 functional categories. For both frequencies, the greatest mRNA response was observed for 1g and 2g.