Fig.2.

Simulated and experimental growth rates as a function of temperature (A) Growth rates relative to the maximum are depicted under each condition. Growth was simulated on minimal medium with glucose (circles) or experimentally measured (diamonds) on Davis minimal medium (DM) with glucose (29), lysogeny broth (LB) (11), or brain heart infusion (BHI) broth (30). The shaded region highlights the temperature range in which the model best predicts relative growth rates. (B) Simulated growth rates relative to maximum WT growth rate are shown for the WT strain, a strain with critical temperatures of the four most growth-limiting proteins at 42.2°C adjusted for maximum activity at that temperature, and a strain with critical temperatures of all growth-limiting proteins at 42.2°C adjusted for maximum activity at that temperature. The gray regions indicate the predicted most growth-limiting proteins and pathways for each phase of WT growth.