Figure 5.
Peptides used in PRE studies. A. Schematic representation of the extracellular hairpin of gp41, with helical residues numbered and shown as rectangles; B. NHR (env5.0) and CHR (C29-e5.0) peptides used to expose ligands binding in the hydrophobic pocket to a paramagnetic relaxation gradient; C. Amino acid sequences of env5.0 and C29-e5.0. Env5.0 includes 4 residues preceding and 3 residues following the gp41 sequence; D. Structural model of C29-e5.0 bound to Fe(env5.0)3 showing a multiconformer representation of the MTSL side chain and representative distances to a bound ligand.