Skip to main content
. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Immunol. 2013 Aug 2;191(5):2072–2081. doi: 10.4049/jimmunol.1300435

Figure 5. TCR-induced CD244 downregulation is rapid, sensitive and independent of PI3K.

Figure 5

A. RSV-YY9 clone was stimulated with cognate peptide for the indicated time, fixed, and stained for expression of CD244 and CD8. Expression was measured by flow cytometry. B. Cultured CD8 T cells were stimulated with PHA and incubated for the indicated time period. Expression of CD244 was not restored over a 48 hour period. C. RSV-YY9 clone was stimulated with increasing doses of cognate peptide overnight prior to fixation and staining. Expression of the indicated markers was measured by flow cytometry. D. RSV-YY9 clone was incubated overnight with or without cognate peptide in the presence of PI3K inhibitor LY294002, and expression of surface markers and IFNγ was measured by ICS. LY294002 effectively inhibited IFNγ production above 30 μM without affecting downregulation of CD244, TIM-3, CD3, or CD8. Results represent at least two experiments.