Figure 3. ILC2 are responsible for IL-5 production and eosinophil accumulation.

(A–D) Rag2^−/− or C57BL/6 mice were infected with PR8 followed by i.p. injection of 500 µg of αThy1.2 depleting antibody at 3 and 5 d.p.i.. Lungs and BAL were analyzed at 7 d.p.i.. (B) Representative FACS plots of lung ILC2 and Thy1.2⁺ cell depletion. (C) BAL IL-5 and (D) absolute number of ILC2 following protocol described in (A). (E) Strategy of depleting ILC2 during the resolution phase via administration of αThy1.2 at 8 d.p.i., followed by i.v. transfer of 2×10⁶ total Thy1.1+ cells (inclusive of ILC2) or CD3⁺Thy1.1⁺ (excluding ILC2) from the lungs of 8 d.p.i. congenic Thy1.1⁺ mice. 100 µg of αThy1.2 was given 9–11 d.p.i. in order to continuously deplete endogenous ILC2. Lungs were harvested at 12 d.p.i. (F) Total eosinophil numbers in the lung following (E). Data is representative of 2–3 independent experiments with n≥4 per group.