Figure 8. CDKs, ERK1/2, and c-Jun are required for efficient MHV68 replication.

(A) 3T3 fibroblasts were treated with vehicle (DMSO), roscovitine, U0126, or 5-iodotubercidin at 10 µM concentration prior to infection with MHV68 at MOI = 10 PFU/cell. Cells were harvested 24 h post-infection, and viral titers were determined by plaque assay. Results are means of triplicate samples. Error bars represent standard deviations. (B) 3T3 fibroblasts were transduced with empty LKO-derived lentivirus or shRNA-encoding lentiviruses to knockdown each of the indicated host proteins. Immunoblot analyses to detect the indicated proteins were performed on cell lysates to evaluate the efficiency of shRNA knockdown. Depleted cells were infected with MHV68 at MOI = 0.05 PFU/cell, and cells were harvested at the indicated times post-infection. Viral titers were determined by plaque assay. Results are means of triplicate samples. Error bars represent standard deviations.