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. 2013 Sep 19;9(9):e1003630. doi: 10.1371/journal.ppat.1003630

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© 2013 Yamamoto-Taguchi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Splenocytes of HBZ-Tg mice or non-Tg mice were stimulated with PMA/ionomycin and protein transport inhibitor for 4 h. IFN-γ, IL-17, TNF-α, IL-4 or IL-2 production was analyzed in CD4⁺ T cells by flow cytometry. (B) Cytokine production was analyzed along with Foxp3 expression. (C) Production of cytokines was shown in CD4⁺Foxp3⁺ T cells and CD4⁺Foxp3⁻ T cells. (D) IFN-γ and Foxp3 expression gated on CD4⁺ T cells from PBMC or cells isolated from the lungs were analyzed by flow cytometry. Percentage of IFN-γ⁺ cells in CD4⁺ splenocytes, PBMC and lung cells. Each symbol represents an individual mouse; small horizontal lines indicate the mean.