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. 2013 Sep 19;8(9):e75436. doi: 10.1371/journal.pone.0075436

Figure 8. Dasatinib and NWASP siRNA abrogate the phenotype in live brain sections.

Figure 8

The term “isolated” is used to describe a glioma cell that is not in contact with other tumor cells. (a) plots the mean velocity (μm/hr) of isolated U251 cells that are the fastest moving in the plane from each of independent fields (20x magnification; a single cell is selected from each field); blue, red, and yellow indicate 5% oxygen (O5, 4 fields), 21% oxygen (O21, 4 fields), and 5% oxygen with 100 nM Dasatinib (D5, 5 fields), respectively. * indicates a two-sample two-tailed ttest P<0.05 as compared to O5. (b) and (c) plot the mean velocity of isolated D54 or U87 cells that are the fastest moving in the plane from each of independent fields (20x magnification; a single cell is selected from each field). In (b) and (c), blue, red, and yellow refer to, respectively, negative control (NC) siRNA under 21% oxygen (NC21; 3 fields for D54 or 4 fields for U87), NC siRNA under 5% oxygen (NC5; 8 fields for D54 or 5 fields for U87), and NWASP siRNA under 5% oxygen (NWASP5, 3 fields for D54 or 4 fields for U87). * indicates a two-sample two-tailed ttest P<0.05 as compared to NC siRNA in NC5. Illustrative time-lapse data are shown in Video S1.