Figure 1. TNF promoter containing the ‘A’ variant of rs361525 has an augmented response to TLR4 and TLR2 ligands, compared to the -238G variant.

Raw 264.7 cells were transiently transfected with firefly-luciferase reporters under the control of the ‘A’ or the ‘G’ variants of the TNF promoter, and co-transfected with a constitutive Renilla-luciferase reporter. Transfected cells were stimulated with the stated concentration of lipopolysaccharide (LPS, TLR-4 ligand; panel A) or Lipoteichoic acid (LTA, TLR-2 ligand; panel B) for 6 hours. A representative of four experiments is shown. Results were analyzed by two-way ANOVA with a Bonferroni post-test. * p<0.05, *** p<0.001, **** p<0.0001.