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. 2013 Sep 19;8(9):e76034. doi: 10.1371/journal.pone.0076034

Figure 3. Overexpression of TR-α induces the activation of the TNF promoter containing the ‘A’ variant of rs361525 in synergy with TLR-4 mediated promoter induction, and is independent of thyroid hormone binding.

Figure 3

Raw 264.7 cells were transiently transfected with firefly-luciferase reporters under the control of the ‘A’ or the ‘G’ variants of the TNF promoter, at position -238 and co-transfected with the stated amount of TR-α expression plasmid (Panel A). Raw 264.7 cells were transiently transfected with the firefly-luciferase reporter under the control of the ‘A’ variant of the TNF promoter, and co-transfected with 30ng of TR-α expression plasmid (Panel B). Transfected cells were stimulated with the stated concentration of lipopolysaccharide (LPS) for 6 hours. Raw 264.7 cells were transiently transfected with the firefly-luciferase reporter under the control of the ‘A’ variant of the TNF promoter and stimulated with the stated concentration of thyroid hormone (TH) and LPS for 6 hours (panels C and D). Results for panels A and B were analyzed by two-way ANOVA with a Bonferroni post-test, for panels C and D by one-way ANOVA with a Bonferroni post-test. *** p<0.001, **** p<0.0001.