Table 1. Nucleotide sequence analysis of the eight MLST loci.

Gene	Locus position (kb)	Lenght (bp) of the sequenced fragment	GC%	S	π/site	Tajima D	Nb of alleles
pepV	431	540	47.0	12 (0)	0.0044	0.136	8
recA	504	399	38.8	30 (3)	0.0265	2.452*	13
glpF	655	399	44.2	13 (1)	0.0091	1.286	12
Tuf	1057	330	39.0	14 (8)	0.0134	1.548	9
dnaK	1214	519	45.1	24 (2)	0.0147	1.513	21
hemN	1220	369	42.2	37 (3)	0.0256	0.894	21
ldhL	1593	441	41.2	9 (3)	0.0046	0.481	9
rpoB	1747	369	39.5	19 (3)	0.0106	0.122	12
Concatenated		3334	41.2	158 (23)	0.0131	1.361	116

Locus position: coordinates in kb based on L. sakei 23K chromosome.

S: number of polymorphic sites (non-synonymous sites are shown in brackets).

π/site : Average pairwise nucleotide difference per site.

D: Tajima's D statistical value based on the population of 232 strains (significant deviation from 0 and from standard neutral model is marked by an *, p<0.05).