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. 2013 Sep 19;8(9):e75729. doi: 10.1371/journal.pone.0075729

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© 2013 Shin et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, Immunofluorescence staining of mDia2 and tubulin in fresh MII oocytes. mDia2, green; α-tubulin, red. B, Immunofluorescence staining of mDia2 and tubulin in oocytes treated with equilibration and vitrification solutions. Note the shrinkage of the ooplasm because of osmosis. mDia2, green; α-tubulin, red.