Figure 4. Effects of ammonia exposure or terrestrial exposure on Na ⁺ :K ⁺ :2Cl ^- cotransporter 1b (nkcc1b) mRNA expression in the brain of Monopterus albus .

Absolute quantification (×10³ copies of transcript per ng of cDNA) of mRNA expression of nkcc1b in the brain of M. albus (A) kept in freshwater (FW; control) or exposed to 50 mmol l^-1 NH₄Cl for 1 day or 6 days, or (B) kept in FW (control) or exposed to terrestrial conditions for 1 day or 6 days. Results represent means + S.E.M. (N=5). Means not sharing the same letter are significantly different (P<0.05).