Figure 1. Systematic genetic analysis of budding yeast Saccharomyces cerevisiae based on the GLAM assay.

GLAM ratios were calculated for the mutants present in the collection of viable deletions, growing in microtiter plates, as described in Materials and methods. The distribution of the values obtained is represented in A. The deletions exhibiting GLAM ratios lower than 0.3 were significantly enriched in the indicated gene ontology category. Those low-scored mutants that maintained low GLAM ratios in exponentially growing cells are listed in B.