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. 2013 Sep 19;9(9):e1003784. doi: 10.1371/journal.pgen.1003784

Figure 9. Meiob is necessary for the maintenance of RAD51/DMC1 recombinase foci during meiotic prophase I.

Figure 9

(A) SYCP3 and RAD51 or DMC1 were detected in chromosome spreads of leptotene, early zygotene, late zygotene, and pachytene/pachytene-like spermatocytes from Meiob +/+ or −/− mice using antibodies that recognized specifically RAD51 or DMC1. RAD51 and DMC1 foci appeared at leptotene stage and disappeared prematurely during the zygotene stage in Meiob −/− spermatocytes. (B) Quantification of RAD51 and DMC1 foci in Meiob+/+ spermatocytes at leptotene (Lepto; n = 10 and 14), early-zygotene (E-Zygo; n = 17 and 15), mid-zygotene (M-Zygo; n = 16 and 18), late-zygotene (L-Zygo; n = 12 and 18) and early-pachytene (E-Pachy; n = 7 and 13) stages and in and Meiob−/− spermatocytes at leptotene (n = 15 and 16), early-zygotene (n = 17 and 21), mid-zygotene (n = 23 and 32) and pachytene-like (Pachy-Like; n = 48 and 43) stages. Median numbers of foci are marked by horizontal lines; ***p<0.0001 (unpaired Student's t-test).