Table 2. Plasmids used in this work.
| Plasmid | Relevant characteristicsa | Reference |
| pKA52 | Overproduction of His6-ParB (based on pET45b(+)) | This study |
| pAH7 | Pnat-parB-YFP, Mx8 attB, (Pnat 1097 bp)b | [39] |
| pAH59 | Pnat-parA-mCherry, Mx8 attB, (Pnat 1097 bp)b | This study |
| pAH73 | PcuoA-parB-YFP, copper-dependent expression of parB-YFP c | This study |
| pNV3 | tetO-array+fragment of mxan0733 region (33°) | This study |
| pMAT20 | tetO-array+fragment of mxan1968 region (90°) | This study |
| pMAT19 | tetO-array+fragment of mxan3779 region (180°) | This study |
| pMAT13 | tetO-array+fragment of mxan4000 region (192°) | This study |
| pMAT18 | tetO-array+fragment of mxan5499 region (270°) | This study |
| pMAT6 | PcuoA-tetR-YFP, Mx8 attB | This study |
| pEB8 | Pnat-ssb-YFP for integration at native site (Pnat 303 bp)d | This study |
| pAH57 | PcuoA-parB, copper-dependent expression of parB, Mx8 attB | This study |
| pAH18 | Construct for in-frame deletion of parB | This study |
| pAH17 | Overproduction of His6-ParA (based on pET45b(+)) | This study |
| pMAT15 | Vector for copper-dependent expressionc | This study |
| pLAU53 | lacI-ECFP and tetR-YFP | [51] |
| pLAU44 | tetO-array (240 copies of 19 bp tetO operator)e | [51] |
a
Promoters for the expression of a particular construct are indicated together with the integration site on the chromosome.
b
The promoter Pnat comprises 1097 bp upstream of the parA-gene.
c
The plasmid integrates at cuoA locus.
d
The promoter Pnat comprises 303 bp upstream of the ssb-gene.
e
The operators are separated by a 10 bp random sequence.