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. 2013 Sep 19;9(9):e1003813. doi: 10.1371/journal.pgen.1003813

Figure 2. Pinpointing the causal mutation affecting ERG28 cis-regulation.

Figure 2

(A) Sequence divergence between RM and BY in the ERG28 promoter region. No other differences exist for 590 bp upstream of the gene, or in the 5′ UTR. (B) Genotypes at the two variable positions for RM, BY, and the two engineered strains. (C) The mRNA levels of ERG28 in each of the two engineered strains compared to wildtype RM, assayed by qPCR. The causal mutation is expected to result in a ∼1.30-fold difference, matching the allelic bias observed in the RM/BY hybrid (Figure 1B), whereas any non-causal mutation will not alter the RM expression level (∼1-fold change). (D) Allelic expression bias in hybrids between each engineered strain and BY, assayed by pyrosequencing. Any non-causal mutation will not alter the 1.30-fold RM/BY allelic bias, whereas the causal mutation is expected to be expressed at the same level as the BY allele (∼1-fold allelic bias).