Figure 7. CIS affects the number and density of MOR SIG particles in PARV-labeled terminals in female, but not male, rats.
Examples of PARV-labeled terminals (PARV-T) containing MOR SIG particles in the cytoplasm (magenta arrows) and on the plasma membrane (magenta arrowhead) from control (A) and CIS (B) estrus females. Scale bars = 500 nm. C. Following CIS, the area of PARV-labeled terminals was significantly less (*p < 0.05) in CIS estrus females compared to controls. D. CIS significantly increased the cytoplasmic density of MOR SIG particles in PARV-labeled terminals of females (**p < 0.01). E. Following CIS, the area of PARV-labeled terminals was not significantly different (p > 0.05) in CIS males compared to controls. F. No significant differences were seen in either the plasma membrane or cytoplasmic density of MOR SIG particles in PARV-labeled terminals of CIS and control males (p > 0.05). N = 3 rats per group; n = 30–50 terminals per rat.