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. Author manuscript; available in PMC: 2014 Sep 17.
Published in final edited form as: Acc Chem Res. 2013 Apr 18;46(9):1933–1941. doi: 10.1021/ar300348n

Figure 8.

Figure 8

Comparison between room temperature and DNP enhanced, low temperature correlation spectra of PI3-SH3. The spectra were obtained with ZF-TEDOR recoupling (τmix = 16 ms) from sample prepared from partially labeled fibrils [15N, 12C] PI3-SH3 /[14N,13C] PI3-SH3 (50:50 molar ratio). (a) 15N-13C intermolecular correlations in PI3-SH3 fibrils at 300 K obtained at 750 MHz in 16 days of acquisition time. (b). Same sample and identical spectral regions were recorded at 100 K and 400 MHz with DNP enhancement in 32 h. (c). Illustration of the 23 interstrand contacts established from 13C -15N peaks in the 750 MHz spectra acquired at 300 K in a. (d) the 52 interstrand contacts established from the 400 MHz DNP enhanced spectra recorded at 100 K shown in (d). 34