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. Author manuscript; available in PMC: 2014 Sep 15.
Published in final edited form as: Respir Physiol Neurobiol. 2013 May 9;188(3):301–307. doi: 10.1016/j.resp.2013.04.024

Figure 2.

Figure 2

Quantification of 5-HT2A receptor protein in tissue micropunches by Western blotting. A: image of a blot double-labeled for 5-HT2A receptor protein and β-actin with two protein samples (lanes) obtained from tissue micropunches. The yellow frames enclose the areas scanned for densitometric measurements of protein amounts in each band. B: staining intensity for each of the two bands measured across the gel shown in A obtained after background subtraction. C: bars representing the ratios of 5-HT2A receptor to β-actin protein amounts for the data illustrated in A and B.