Table 1.
Neurotransmitter receptor mRNA levels quantified by RT-PCR near the time of the rest period onset (8–9 am) and near the active period onset (6–7 pm) in the XII nucleus and external cuneate nucleus (ECN).
| Transcript and its accession number | Transcript copy numbers* ±SE | |||||
|---|---|---|---|---|---|---|
| XII nucleus | ECN | |||||
| 8–9 am | 6–7 pm | p value | 8–9 am | 6–7 pm | p value | |
| 5-HT2A M30705 | 5.2±2.8 | 14.9±3.7 | 0.03 | 0.7±0.3 | 1.8±0.8 | 0.09 |
|
| ||||||
| 5-HT2C M21410 | 49.7±14.0 | 42.4±10.0 | 0.82 | 8.2±3.5 | 7.3±2.4 | 0.64 |
|
| ||||||
| α1AAR U07126 | 63.5±18.1 | 44.8±8.1 | 0.38 | 41.4±13.6 | 67.9±17.1 | 0.12 |
|
| ||||||
| α1BAR L08609 | 20.0±2.9 | 23.1±5.2 | 0.59 | 6.5±1.4 | 13.4±3.0 | 0.06 |
|
| ||||||
| ORX 2R AF041246 | 1291±476 | 711±175 | 0.77 | 807±255 | 1656±1038 | 0.85 |
|
| ||||||
| Tubulin, α1NM_022298 (absolute) | 9426±2191 | 11864±3277 | 0.54 | 3767±913 | 3496±1064 | 0.77 |
*
Receptor mRNA levels were quantified at each time point and anatomical location as the number of cDNA copies of the target cDNA per 1,000 copies of the housekeeping gene, tubulin, in the same sample. The bottom row shows the mean absolute cDNA copy numbers per sample for tubulin. p values describe for each transcript the statistical significance of the difference between the samples collected at the two different time points from the XII nucleus or the ECN (n=7–9 rats per group).