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. Author manuscript; available in PMC: 2014 Sep 15.
Published in final edited form as: Clin Cancer Res. 2013 Sep 10;19(18):4941–4950. doi: 10.1158/1078-0432.CCR-13-1253

Figure 1.

Figure 1

Validation of Nanostring Data. Nanostring counts for CSPG4 gene expression in five melanoma lines were graphed against results from RT-PCR analysis (A) and MFI from FACS with CSPG4-specific mAb (B). Immunohistochemistry results, including scores for intensity of staining (C) and percentage of tumors cells staining (D) for NY-ESO-1 staining in 41 tumors samples are graphed against the log of the Nanostring counts for NY-ESO-1. Immunohistochemistry scores are as follows: for intensity of staining 0=no reactivity, 1=weak reactivity, 2=moderate reactivity, 3=intense reactivity, and for percentage of tumors cells that stained 0=0%, 1=0-5%, 2=5-50%, 3=>50%.