Abstract
The production of extracellular deoxyribonuclease and ribonuclease by 23 marine and 3 dairy strains of Pseudomonas putrefaciens, 15 strains of fish-pathogenic fluorescent pseudomonads, 38 strains of fluorescent pseudomonads isolated from haddock, and 34 related organisms was determined by an agar plate method. All strains of P. putrefaciens produced both deoxyribonuclease and ribonuclease. Of the other 87 organisms examined, 26.5% produced ribonuclease and 14.5% produced deoxyribonuclease. All organisms which produced deoxyribonuclease also produced ribonuclease. Deoxyribonuclease production by P. putrefaciens is suggested as a useful criterion of identity for members of this intense fish spoilage species.
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- Hughes R. B., Jones N. R. Measurement of hypoxanthine concentration in canned herring as an index of the freshness of the raw material, with a comment on flavour relations. J Sci Food Agric. 1966 Sep;17(9):434–436. doi: 10.1002/jsfa.2740170911. [DOI] [PubMed] [Google Scholar]
- JEFFRIES C. D., HOLTMAN D. F., GUSE D. G. Rapid method for determining the activity of microorganisms on nucleic acids. J Bacteriol. 1957 Apr;73(4):590–591. doi: 10.1128/jb.73.4.590-591.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Levin R. E. Detection and incidence of specific species of spoilage bacteria on fish. I. Methodology. Appl Microbiol. 1968 Nov;16(11):1734–1737. doi: 10.1128/am.16.11.1734-1737.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Martin W. J., Ewing W. H. The deoxyribonuclease test as applied to certain gram-negative bacteria. Can J Microbiol. 1967 May;13(5):616–618. doi: 10.1139/m67-080. [DOI] [PubMed] [Google Scholar]
- Papaevangelou G., Papavassiliou J. Comparison of desoxyribonuclease activity to some other criteria of Staphylococcus pathogenicity. Pathol Microbiol (Basel) 1967;30(1):59–63. doi: 10.1159/000161644. [DOI] [PubMed] [Google Scholar]
- Rothberg N. W., Swartz M. N. Extracellular Deoxyribonucleases in Members of the Family Enterobacteriaceae. J Bacteriol. 1965 Jul;90(1):294–295. doi: 10.1128/jb.90.1.294-295.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Valu J. A. Use of the deoxyribonuclease test as an aid in the differentiation of Paracolobactrum (Hafnia) from serratia. J Bacteriol. 1966 Jan;91(1):467–468. doi: 10.1128/jb.91.1.467-468.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]