Figure 1. Baseline surface CXCR4 levels affect the degree of chemotaxis toward an SDF-1α gradient.
(a) Surface CXCR4 expression in viable, untreated cells was measured by flow cytometry. Results are the averages of triplicate measurements. Error bars in this and subsequent figures represent SEM. (b) MOLM-14 and Nalm-6 were seeded in hanging cell culture inserts and placed into 24-well plates containing supplemented media alone (control) or supplemented media with SDF-1α (75 or 150 ng/mL). Migrated cells were measured in triplicate after 4 and 24 hours. *p<0.05 vs. control.