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. Author manuscript; available in PMC: 2014 May 21.
Published in final edited form as: Biochemistry. 2013 May 9;52(20):10.1021/bi400047u. doi: 10.1021/bi400047u

Figure 1.

Figure 1

p21 promotes D1/K2 complex assembly. (A) Immunoblot analysis of crude lysates and affinity purified D1/K2 complexes from p21−/− HCT116 cells infected with adenoviruses encoding Cyclin D1, Cdk2, and increasing amounts of p21. (B) Silver stained SDS-PAGE gel of affinity purified D1/K2 complexes from p21−/− HCT116 cells infected with adenoviruses encoding Cyclin D1, Cdk2, and increasing amounts of p21. (C) Silver stained SDS-PAGE gel of affinity purified D1/K2 complexes from p21−/− HCT116 cells infected with adenoviruses encoding Cyclin D1, Cdk2, and the indicated p21 constructs. (D) Immunoblot analysis of affinity purified D1/K2 complexes from p21−/− HCT116 cells infected with adenoviruses encoding Cyclin D1, Cdk2, and the indicated p21 constructs. (E) Immunoblot analysis of affinity purified Cyclin D1 complexes from p21−/− HCT116 cells infected with adenoviruses encoding the indicated proteins. (F) Model of trimeric p21/PCNA complexes containing multiple Cyclin/Cdk complexes.