Skip to main content
. Author manuscript; available in PMC: 2014 May 21.
Published in final edited form as: Biochemistry. 2013 May 9;52(20):10.1021/bi400047u. doi: 10.1021/bi400047u

Figure 3.

Figure 3

D1/K2 complexes and the Cyclin D1-Cdk2 fusion protein phosphorylate NPM. (A) Immunoblot analysis of crude lysates (left panel) and affinity purified Cyclin/Cdk complexes (right panel) from NMuMG cells transduced with adenoviruses encoding the indicated proteins. (B) Immunoblot analysis of an in vitro kinase assay using Cyclin/Cdk fusion proteins purified from 293T cells transiently transfected with vectors encoding the indicated proteins. HC represents the heavy chain of the antibody used in the immunoprecipitation. The band labeled Rb below is the GST-Rb substrate present in the kinase assays. (C) Immunoblot analysis of cell lysates obtained from 293T cells transiently transfected with constructs coding for the indicated proteins. “(GFP-NPM)” indicates that the bands shown represent the exogenously expressed GFP-NPM rather than endogenous NPM.