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. Author manuscript; available in PMC: 2014 May 21.
Published in final edited form as: Biochemistry. 2013 May 9;52(20):10.1021/bi400047u. doi: 10.1021/bi400047u

Figure 6.

Figure 6

Analysis of PSF phosphorylation. (A) Immunoblot analysis of FLAG-PSF or non-phosphorylatable PSF(12M) expressed with different Cyclin/Cdk complexes. (B) Immunoblot analysis of FLAG-PSF containing various mutations of potential phosphorylation sites expressed with D1K2. (C) Autoradiography of His6-PSF [γ-32P]-phosphorylated with different Cyclin/Cdk complexes. (D) Autoradiography of His6-PSF [γ-32P]-phosphorylated with either Cyclin E/Cdk2 or D1K2 (left panel) and quantitation of the same bands using a scintillation counter (right panel).