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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: FEBS Lett. 2013 Aug 28;587(19):3243–3248. doi: 10.1016/j.febslet.2013.08.018

Figure 1.

Figure 1

Relative fluorescence emission of sfGFP in twenty natural amino acids with FRS variants. The y-axis units are the percentages of fluorescence intensity from sfGFP with Ser2TAG mutation compared to the fluorescence intensity of wild type sfGFP. The fluorescence intensity was measured by excitation wavelength in 485 nm/20 nm and emission wavelength 528 nm/20 nm. Each fluorescence intensity was presented after subtraction with the value from the control experiment without adding the amino acid.