. Author manuscript; available in PMC: 2014 Oct 1.

Published in final edited form as: FEBS Lett. 2013 Aug 28;587(19):3243–3248. doi: 10.1016/j.febslet.2013.08.018

Table 2.

ATP-PPi exchange kinetics of PylRS variants with Phe^a

Enzyme	K_m (mM)	k_cat (min⁻¹)	Reference
FRS1	21.0 ± 3.3	2.2 ± 0.2	This work
FRS2	14.0 ± 1.5	3.7 ± 0.1	This work
FRS3	22.8 ± 3.8	4.5 ± 0.3	This work
M. barkeri PylRS	0.055 ± 0.005	6.3 ± 0.7	[18]
E. coli PheRS	0.040	8280	[17]

The values were calculated from three independent experiments.