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. 2013 Sep 1;27(17):1903–1916. doi: 10.1101/gad.219899.113

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© 2013, Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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Figure 2. — PRMT5 is required for NPC homeostasis. (A) Number of primary neurospheres and total number of cells from cultures of E14.5 dorsal telencephalon NPCs derived from Prmt5^F/F and Prmt5^F/FNes embryos. Each bar represents an average of at least three experiments. (B) Number of secondary neurospheres, as in A. (C) Primary neurospheres (left panel) from Prmt5^F/FNes mice infected with empty vector (EV), wild-type PRMT5 (hPRMT5), or a catalytically inactive PRMT5 mutant (hPRMT5AAA) and passaged to derive secondary and tertiary neurospheres (right panel). (D) Neurospheres derived from Prmt5^F/F or Prmt5^F/FNes NPCs were stained with DAPI and CC3, and the percentage of pyknotic nuclei was counted. (E) Protein levels upon treatment with OHT and subsequent PRMT5 depletion for 4 d. The antibodies used are indicated on the right of each panel. As a positive control, p53 and the DDR were induced by treating cells with 10 μM etoposide for 2 h.